Overview:

GowiththeflowforeasyexosomeisolationusingFACS

TheCD63Exo-FlowCaptureKithasallthereagentsyouneedtopurifyexosomesusingFACS—magneticstreptavidinbeads,CD63-biotincaptureantibody,washandelutionbuffers,andreversIBLeExo-FITCstain(Cat.#EXOFLOW800A-1)—yousupplytheFACSandtheoptionalmagneticstand(Cat.#EXOFLOW700A-1).Ourwell-validatedCD63-biotinantibodyandhigh-qualitykitcomponentsensurereliable,reproducibleexosomepurificationbasedonthepresenceofCD63ontheexosomesurface.Andwithourlarger-than-typicalbeadsize(9.1μmdiameter)exosomecaptureishighlyefficient,assistingwithrecoveryofrareexosomepopulations.

HowItWorks:

EasilypurifyexosomesusingFACSwiththeCD63Exo-FlowCaptureKit

Ataglance
Simply(1)coupletheCD63-biotinantibodytothemagneticstreptavidinbeads,(2)usetheCD63-coupledmagneticbeadstocaptureexosomesthathavebeenisolatedusingeitherExoQuick®orultracentrifugation,(3)washawayunboundexosomes,andthen(4)stainwithreversibleExo-FITC(excitationandemissionwavelengthsof494nmand518nm,respectively).

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