- Rigorouslyvalidated
- Hand-pickedselection
- Expertexosomesupport
Choosetherightantibodyforyourexosomedetectionneeds
Cat.# | Antibodyto | Notes | LinktoExoCartaentryforthehumanprotein |
---|
Generalexosomemarkers |
EXOAB-CD9A-1 | CD9 | Tetraspanin | ExoCarta_928 |
EXOAB-CD63A-1 | CD63 | Tetraspanin | ExoCarta_967 |
EXOAB-CD81A-1 | CD81 | Tetraspanin | ExoCarta_93185 |
EXOAB-Hsp70A-1 | HSP70 | | ExoCarta_3303,ExoCarta_3304,ExoCarta_3306,ExoCarta_3308,ExoCarta3310,ExoCarta_3311 |
EXOAB-ALIX-1 | ALIX | Programmedcelldeath6interactingprotein(PDCD6IP) | ExoCarta_10015 |
EXOAB-ANXA5-1 | ANXA5-1 | AnnexinA5 | ExoCarta_308 |
EXOAB-TSG101-1 | TSG101 | Tumorsusceptibilitygene101 | ExoCarta_7251 |
EXOAB-FLOT1-1 | FLOT-1 | Flotillin-1 | ExoCarta_10211 |
Tissue-specificexosomemarkers |
EXOAB-CXCR4-1 | CXCR4 | SpecificforB-cells,T-cells,andplatelets | ExoCarta_7852 |
EXOAB-EPCAM1-1 | EpCam | Specificforcancer-derivedexosomes | ExoCarta_4072 |
EXOAB-VMTN-1 | Vimentin | Specificformesenchymalstemcells | ExoCarta_7431 |
SeeisolationdatausingExo-FLOWIPKits
Forthesestudies,eitherhumanserumorHEK293exosomesconcentratedfromcellculturemediausingExoQuick-TCwereaddedtotheantibody-coupledmagneticbeads.Afterwashing,exosomeswereelutedandrecoveryestimatedusingastandardBCAproteinassay.
Figure1.CD63andCD9,twoexosomemarkers,arereADIlydetectedinsamplespurifiedusingExo-FlowKits.Approximately1µgofproteinwasloadedperwellona4-20%gradientproteinPAGE.TheproteinswereseparatedandtransferredtonitrocellulosemembranesforWesternblotanalysis.Theblotswereprobedwitheitherwithanti-CD63oranti-CD9antibodiestodetecttheexosomeproteinmarkers.
Figure2.NanoSightanalysisshowsExo-FlowIPKitsdelivergoodyieldsofparticleswhosesizesareconsistentwithexosomes.
品牌介绍
美国SBI代理
System Biosciences,简称SBI,美国加州湾区新成立的生技公司,致力于独特、创新生物技术之开发,以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰(RNAi)研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术,为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司,提供基于慢病毒
美国SBI代理
ExoQuick and ExoQuick-TC Products
Product
Size
Catalog #
ExoQuick serum exosome precipitation solution (5 ml)
75 reactions
EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product
75 reactions
EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product
100 reactions
TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)
300 reactions
EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)
10 reactions
EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)
50 reactions
EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified
50 ml
EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified
250 ml
EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit
5 reactions
EXOLP5A-1