Overview

Monitor the balance of T cell dynamics in autoimmunity, inflammation, and cancer

Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With the Mouse Foxp3 Promoter GreenFire Lentivector you can combine SBI’s well-regarded and high quality lentivector technology with a Foxp3 promoter-reporter architecture that enables easy and quantitative monitoring of Foxp3 expression. The Foxp3 promoter drives coordinated expression of GFP and luciferase reporters.

The promoter sequences and design are based upon Tone and Greene¹, Zheng, et al.², and Burgler, et al³.

References

1. Tone, M and Greene, MI. Cooperative regulatory events and Foxp3 expression. Nat Immunol. 2011 Jan; 12(1): 14–16. PMCID: PMC3151723.
2. Zheng Y, et al. Role of conserved non-coding DNA elements in the Foxp3 gene in regulatory T-cell fate. Nature. 2010 Feb 11; 463(7282):808-12. PMCID: PMC2884187.
3. Burgler, S, et al. RORC2 is involved in T cell polarization through interaction with the FOXP3 promoter. J Immunol. 2010 Jun 1; 184(11):6161-9. PMID: 20427770.

Supporting Data

Luciferase activation in response to Foxp3 transcriptional activators

Figure 1. SBI’s GreenFire Lentivector reporters for mFoxp3, mRORγt, and mIL-17 show strong luciferase activity in response to known transcriptional activators. METHODS: Human Jurkat T cells were transduced with lentivirus for the Foxp3, RORγt and IL-17 promoter reporters. Transcription activation was tested using stimulation through the addition of with PMA (5 ng/ml) and Ionomycin (500 ng/ml). Cells were harvested after 24 hours and luciferase activity measured for transcription activation responses.

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