Overview

Delivering ELISA-based exosome quantitation ULTRA fast

Improving on our popular ExoELISA Kits, the ExoELISA-ULTRA CD81 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.

Currently configured for detection of CD81, a common exosomal marker, ExoELISA-ULTRA CD81 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with common biofluids. The ExoELISA-ULTRA CD81 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NTA analysis and enables quantitation of exosomes carrying CD81 in your target samples. One ExoELISA-ULTRA CD81 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.

• Sensitive—detect as little as 1 µg protein equivalent
• Fast—complete in less than 4-hours—no more overnight incubation
• Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
• Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD81
• Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications

Choose the exosome quantitation method that’s best for your studies

Supporting Data

The standard curve for ExoELISA-ULTRA CD81 shows robust linearity down to ~4 x 10⁹ exosomes.

Figure 1. ExoELISA-ULTRA CD81 standard curve shows robust linearity down to ~4 x 10⁹ exosomes. OD450nm values of commonly used samples (e.g. human serum, HEK293T, and MCF7 breast cancer exosomes – in large colored dots) fall well within the standard curve for the assay.

Resources

Citations

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