>
产品中心 >
RNA_interference >
SBI/FluoroCet Exosome Quantitation Kit/96 Reactions/FCET96A-1-96 Reactions
Overview
Get ultrasensitive exosome quantitation when samples are limiting
Enabling highly sensitive quantitation of exosomes in only 60 minutes, the FluoroCet™ Kit takes our popular EXOCET assay and increases the sensitivity over 30-fold. Fully compatible with all of SBI’s ExoQuick® exosome isolation reagents as well as ultracentrifugation, FluoroCet is great for quantifying exosomes when samples are limiting.
- Sensitive
- Requires as little as 500 ng of exosomal protein
- Perfect for clinical samples or any situation where samples are limiting
- Fast—assay is complete in only 60 minutes
- Versatile—compatible with all major exosome isolation methods (e.g. ExoQuick, ultracentrifugation, ultrafiltration, and immunoffinity capture) from human, mouse, and rat samples
- Quantitative—internal assay standards enable calculation of exosome abundance
Like EXOCET, FluoroCet measures the activity of acetecylcholinesterase (AChE), a known exosome protein, although the more sensitive FluoroCet assay relies on readout of fluorescence emission at 590-600 nm. To enable calculation of exosome abundance in your target sample, each kit includes standards calibrated to known amounts of human exosomes as determined by NanoSight analysis. The FluoroCet Kit contains all of the reagents needed to perform 96 reactions.
Choose the exosome quantitation method that’s best for your studies
| ExoELISA-ULTRA CD63ExoELISA-ULTRA CD81 | ExoELISA CD9ExoELISA CD63ExoELISA CD81 | EXOCET | FluoroCet | |
|---|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For sensitive quantitation of exosomes when time and input sample are not limiting | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 24 hours | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | >500 µg | 50 µg | <1> |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 | ExoELISA CD9ExoELISA CD63ExoELISA CD81 | EXOCET | FluoroCet |
How It Works
Exosome quantitation with FluoroCet is quick and easy
- Calibrate your fluorescence plate reader
- Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
- Lyse exosomes with the included Exosome Lysis Buffer
- Measure AChE activity—add the included buffer and incubate for 20 minutes (or appropriate time as determined during the calibration step) at room temperature in the dark, and then measure fluorescence using excitation at 530–570 nm and emission at 590–600 nm.
Supporting Data
The sensitive FluoroCet exosome quantitation assay
Data from FluoroCet remains linear—and quantitative—even at low exosome amounts. Comparison of limit of detection (LOD) of EXOCET versus FluoroCet.
