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SBI/XPack MSCV-XP-MCS-EF1α-Puro Cloning Lentivector/10 µg/XPAK710PA-1-10 µg
Overview
Turn your protein into exosome cargo with XPack™
Whether you’re engineering exosomes as protein delivery vehicles, labeling exosomes with a custom reporter protein, or performing functional studies, XPack gives you the power to package your protein-of-interest into exosomes.
XPack MSCV-XP-MCS-EF1α-Puro Cloning Lentivector
With the XPack MSCV-XP-MCS-EF1α-Puro Cloning Lentivector, you can use SBI’s well-proven lentivector technology to express your protein-of-interest and package it into exosomes. The strong MSCV promoter delivers high expression levels in hematopoietic and stem cell lines and the multiple cloning site downstream of the XPack tag enables fusion of the exosome-targeting peptide to your protein-of-interest (also available with a CMV promoter for expression in hematopoietic and stem cells).
The XPack System at a glance:
- Powerful—enables a wealth of applications, including
- Exosome labeling
- Exosome cargo tracking
- Exosomes as cargo delivery vehicles
- Exosomes as biotherapeutics
- Exosome-generating stable cell lines with protein cargo-of-interest
- Optimized—SBI developed the XPack peptide tag for robust exosome targeting
- Reliable—based on SBI’s highly-cited, well-proven lentivector technology
- Versatile
- Two promoter options for expression in a wide range of cells
- Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors
Choose the XPack Lentivector that’s right for you
| CMV Promoter | (For most cell lines) | MSCV Promoter | (For hematopoietic and stem cell lines) | |
|---|---|---|---|---|
| Customize with our cloning vector | ||||
| XPAK510PA-1 | XPack CMV-XP-MCS-EF1α-Puro | XPAK710PA-1 | XPack MSCV-XP-MCS-EF1α-Puro | |
| Label exosomes with pre-built reporters | ||||
| GFP | XPAK530CL-1 XPAK530PA-1 XPAK530VA-1 | XPack CMV-XP-GFP-EF1α-Puro | XPAK730PA-1 XPAK730VA-1 | XPack MSCV-XP-GFP-EF1α-Puro |
| RFP | XPAK531PA-1 | XPack CMV-XP-RFP-EF1α-Puro | XPAK731PA-1 | XPack MSCV-XP-RFP-EF1α-Puro |
| Luciferase | XPAK532CL-1 XPAK532PA-1 XPAK532VA-1 | XPack CMV-XP-Luciferase-EF1α-Puro | XPAK732PA-1 XPAK732VA-1 | XPack MSCV-XP-Luciferase-EF1α-Puro |
How It Works
Optimized for success
With XPack, SBI has developed an optimized N-terminal peptide sequence that targets the peptide—and any protein fused to it—to the inner surface of the exosome membrane. Clone your protein of interest into one of the XPack Cloning Vectors and, when expressed, your protein will be packaged into exosomes.
Supporting Data
Engineer exosomes with XPack and deliver functional protein to cells, changing cellular physiology
Cyclin-dependent kinase inhibitor 1B (CDKN1B) is a 22 KDa protein which is a cell cycle inhibitor and tumor suppressor. To demonstrate how exosomes can be used to deliver functional protein to cells, we generated exosomes containing CDKN1B and show that addition of these exosomes HEK-293 cells can reduce cellular proliferation (Figure 1).
Figure 1. XPack-CDKN1B exosomes reduce cellular proliferation. METHODS: We cloned the CDKN1B gene into CMV-XP-MCS-EF1α-Puro Cloning Lentivector (Cat.# XPAK510PA-1), transfected this construct into HEK293 cells, and isolated exosomes after 48 hours using ExoQuick-TC®. We then added 5, 50, or 100 µg of XPack-CDKN1B exosomes to approximately 5000 HEK-293 target cells in 96-well tissue culture plates at 24-hour intervals over the course of 72 hours. Exosomes isolated from non-transfected cells were used for controls. Cellular proliferation was quantified using an MTT Assay (Millipore catalog # CT02) and normalilzed to HEK-293 cells treated with control exosomes. XPack-CDKN1B exosomes consistently reduce the rate of cellular proliferation.
