>
产品中心 >
RNA_interference >
SBI/All-in-one Cas9 SmartNucleaase&trade&；AAVS1-gRNA质粒/10µg/CAS601A-1-10µg
Overview
Streamline genome editing at the powerful AAVS1 Safe Harbor Site
When you have transfectable cells and want to take advantage of the robust and reliable expression that’s possible from the AAVS1 Safe Harbor Site, SBI offers a streamlined plasmid system that’s ready to go. The All-in-one Cas9 SmartNuclease™ & AAVS1 gRNA Plasmid expresses both Cas9 and an AAVS1-targeting gRNA—just co-transfect with a homologous recombination (HR) Targeting Vector and select for your desired colonies.
Delivering consistent, robust transgene expression, the AAVS1 safe harbor site is a preferred target for gene knock-ins. Insertion at the site has been shown to be safe with no phenotypic effects reported, and the surrounding DNA appears to be kept in an open confirmation, enabling stable expression of a variety of transgenes.
SBI’s AAVS1 Safe Harbor Targeting products deliver:
- Easy, precise knock-in of any gene
- Consistent, robust transgene expression from the AAVS1 Safe Harbor Site
- Simplified construction of isogenic cell lines
- Minimal off-target integration when using our AAVS1 HR Targeting Vectors
- Streamlined CRISPR/Cas9 library screening
Note that we’ve specially designed a series of AAVS1 HR Targeting Vectors that greatly reduce off-target integration events (Cat.# GE620A-1, GE622A-1, and GE624A-1). Taking advantage of the AAVS1’s location within an intron, these vectors come with a puromycin marker that has no promoter, only a splice acceptor site—expression of puromycin can only occur when the construct integrates within an intron, reducing the probability of recovering off-target integrants in the presence of puromycin selection.
As with all of our Cas9 delivery options, the All-in-one Cas9 SmartNuclease & AAVS1 gRNA Plasmid is functionally validated and comes backed by our expert technical support team—if you’ve got a genome engineering question just ask by emailing tech@systembio.com.
Not sure whether you need a CRISPR/Cas9 plasmid, purified protein, or mRNA?
Use this table to choose the CRISPR/Cas9 product that’s right for you:
For This Application | In these types of cells |
|---|
Use These Products
- Gene tagging
- Transgenic organism generation
- Model organism engineering
- Stable KO, KI, and genome editing of somatic cells
- Transgenic cell line generation
- Cell-based disease models
- Primary cells
- Hematopoietic cells
- Stem cells
- Genome-wide surveys
- gRNA library screens
- Functional screens
- Off-target events are of highest concern
