Overview
Simplify large scale lentivirus production with the LentiSuite Basic Kit
Get the reagents you need for large scale lentivirus production efficiently bundled into a single kit with the LentiSuite Basic Kit. This kit is ideal for fast-moving labs that don’t need quantitative titer information, and supports generation of high-titer lentivirus particles for any application where stable gene delivery and expression is desired.
Compatible with all of SBI’s lentivectors and constructs, as well as all other third-generation lentivectors, the LentiSuite Basic Kit includes optimized reagents to support large-scale virus production—you provide the HEK293T/TN/FT producer cells and lentivector (or purchase them separately) and this kit provides enough critical reagents to support 10 virus preps from 10 cm plates, or 5 virus preps from 15 cm plates:
- pPACK Packaging System (200 µL)
- PureFection Transfection Reagent (1 mL)
- PEG-it Concentration Reagent (100 mL)
- TransDux™ MAX Transduction Reagent (100 reactions*)
With the LentiSuite Basic Kit, you get more than the kit—you get confidence backed by:
- SBI’s well-proven, widely-published lentivirus technology
- Optimized reagents that we keep on improving
- Deep technical expertise from the scientists that develop our lentivirus production products and perform our lentivirus production services
*One transduction reaction is defined as 500 µL of total volume in a single well of a 24-well plate.
How It Works
For fast-moving labs generating lentivirus at large scales
Kit Contents
pPACK Packaging System
- Reliable—generate high titers of transduction-ready pseudoviral particles
- Powerful—efficiently deliver and stably express your lentivector constructs in a wide range of mammalian cells
- Well-designed—quantify pseudoviral titers and transduction efficiency using a convenient copGFP control
- Flexible—choose from FIV and HIV-based lentivector packaging kits
PureFection Transfection Reagent
- Flexible—highly effective transfection technology supports a range of applications
- Compatible with most cell types
- Works with both plasmid DNA and siRNAs
- Cost-effective—excellent alternative to expensive, lipid-based products
- Safe—nano-based gene delivery with low toxicity
- Fast—quick fifteen-minute protocol makes PureFection ideal for high-throughput transfections
PEG-it Concentration Reagent
- Easy-to-use—simplifies lentiviral production by removing the need for ultracentrifugation
- Cost-effective—contributes to more efficient transductions
- Powerful—concentrate lentiviral particles 10- to 100-fold
- Scalable—enables isolation of virus from large volumes of media
- Safe—supports a range of applications
- Found to be non-toxic to all target cell lines tested, including ES cells
- Prevent toxic and immunogenic reactions by avoiding accumulation of cellular debris
TransDux™ MAX Transduction Reagent
- Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR*
- Easy-to-use—kit format requires minimal hands-on time (<5 minutes)
- Non-toxic—no need to change out media after infection
- Versatile—works with all types of packaged lentivirus
- Flexible—enhances transduction efficiency in a wide variety of cell lines
- Broadly compatible—does not interfere with downstream gene expression or functional assays
*Based on infection of HT1080 cells with the pre-packaged virus format of SBI’s BLIV 2.0 reporter MSCV-Luciferase-EF1a-copGFP-T2A-Puro (Cat# BLIV713VA-1) and the appropriate conditions for polybrene, TransDux, and TransDux MAX. Virus transduction & integration efficiency was measured using SBI’s UltraRapid Titering Kit (Cat #LV961A-1).
