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当前位置: 首页 > 产品中心 > RNA_interference > SBI/LentiSuite基本套件/10 Preps/LV340A-1-10 Preps
商品详细SBI/LentiSuite基本套件/10 Preps/LV340A-1-10 Preps
SBI/LentiSuite基本套件/10 Preps/LV340A-1-10 Preps
SBI/LentiSuite基本套件/10 Preps/LV340A-1-10 Preps
商品编号: LV340A-1-10Preps
品牌: System Biosciences
市场价: ¥19900.00
美元价: 11940.00
产地: 美国(厂家直采)
公司:
产品分类: RNA干扰
公司分类: RNA_interference
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

Simplify large scale lentivirus production with the LentiSuite Basic Kit

Get the reagents you need for large scale lentivirus production efficiently bundled into a single kit with the LentiSuite Basic Kit. This kit is ideal for fast-moving labs that don’t need quantitative titer information, and supports generation of high-titer lentivirus particles for any application where stable gene delivery and expression is desired.

Compatible with all of SBI’s lentivectors and constructs, as well as all other third-generation lentivectors, the LentiSuite Basic Kit includes optimized reagents to support large-scale virus production—you provide the HEK293T/TN/FT producer cells and lentivector (or purchase them separately) and this kit provides enough critical reagents to support 10 virus preps from 10 cm plates, or 5 virus preps from 15 cm plates:

  • pPACK Packaging System (200 µL)
  • PureFection Transfection Reagent (1 mL)
  • PEG-it Concentration Reagent (100 mL)
  • TransDux™ MAX Transduction Reagent (100 reactions*)

With the LentiSuite Basic Kit, you get more than the kit—you get confidence backed by:

  • SBI’s well-proven, widely-published lentivirus technology
  • Optimized reagents that we keep on improving
  • Deep technical expertise from the scientists that develop our lentivirus production products and perform our lentivirus production services

*One transduction reaction is defined as 500 µL of total volume in a single well of a 24-well plate.

How It Works

For fast-moving labs generating lentivirus at large scales

Kit Contents

pPACK Packaging System

  • Reliable—generate high titers of transduction-ready pseudoviral particles
  • Powerful—efficiently deliver and stably express your lentivector constructs in a wide range of mammalian cells
  • Well-designed—quantify pseudoviral titers and transduction efficiency using a convenient copGFP control
  • Flexible—choose from FIV and HIV-based lentivector packaging kits

PureFection Transfection Reagent

  • Flexible—highly effective transfection technology supports a range of applications
    • Compatible with most cell types
    • Works with both plasmid DNA and siRNAs
  • Cost-effective—excellent alternative to expensive, lipid-based products
  • Safe—nano-based gene delivery with low toxicity
  • Fast—quick fifteen-minute protocol makes PureFection ideal for high-throughput transfections

PEG-it Concentration Reagent

  • Easy-to-use—simplifies lentiviral production by removing the need for ultracentrifugation
  • Cost-effective—contributes to more efficient transductions
  • Powerful—concentrate lentiviral particles 10- to 100-fold
  • Scalable—enables isolation of virus from large volumes of media
  • Safe—supports a range of applications
    • Found to be non-toxic to all target cell lines tested, including ES cells
    • Prevent toxic and immunogenic reactions by avoiding accumulation of cellular debris

TransDux™ MAX Transduction Reagent

  • Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR*
  • Easy-to-use—kit format requires minimal hands-on time (<5 minutes)
  • Non-toxic—no need to change out media after infection
  • Versatile—works with all types of packaged lentivirus
  • Flexible—enhances transduction efficiency in a wide variety of cell lines
  • Broadly compatible—does not interfere with downstream gene expression or functional assays

*Based on infection of HT1080 cells with the pre-packaged virus format of SBI’s BLIV 2.0 reporter MSCV-Luciferase-EF1a-copGFP-T2A-Puro (Cat# BLIV713VA-1) and the appropriate conditions for polybrene, TransDux, and TransDux MAX. Virus transduction & integration efficiency was measured using SBI’s UltraRapid Titering Kit (Cat #LV961A-1).

Resources

User Manual: LentiSuite Basic Kit
Lentivirus Safety Guidelines
Brochure: Gene Delivery and Expression Products and Services
品牌介绍
美国SBI代理 System Biosciences,简称SBI,美国加州湾区新成立的生技公司,致力于独特、创新生物技术之开发,以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰(RNAi)研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术,为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司,提供基于慢病毒 美国SBI代理 ExoQuick and ExoQuick-TC Products Product Size Catalog # ExoQuick serum exosome precipitation solution (5 ml) 75 reactions EXOQ5A-1 ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product 75 reactions EXOQ5TM-1 Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product 100 reactions TMEXO-1 ExoQuick serum exosome precipitation solution (20 ml) 300 reactions EXOQ20A-1 ExoQuick-TC for Tissue Culture Media and Urine (10ml) 10 reactions EXOTC10A-1 ExoQuick-TC for Tissue Culture Media and Urine (50ml) 50 reactions EXOTC50A-1 Exosome-depleted FBS Media Supplement - USA Certified 50 ml EXO-FBS-50A-1 Exosome-depleted FBS Media Supplement - USA Certified 250 ml EXO-FBS-250A-1 ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit 5 reactions EXOLP5A-1
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