Overview
Increase the infectivity of any retrovirus by concentrating with Retro-Concentin
Quantitatively precipitate any retrovirus with Retro-Concentin™. Instead of using lengthy spins in an ultracentrifuge, Retro-Concentin enables concentration of retroviruses directly from culture medium with a simple one (or an optional two) low-speed centrifugation steps.
In addition, Retro-Concentin stabilizes the retroviruses for frozen storage, providing an additional advantage over other concentration methods.
Each preparation can handle up to 200 mL of retroviral supernatant, which is compatible with most centrifuges, and the resulting pellet can be dissolved in the volume of buffer that meets your experimental requirements.
With Retro-Concentin, you get simple and effective retrovirus concentration:
- Fast protocol
- Cost-effective
- Up to 100-fold concentration of retrovirus particles
How It Works
Retro-Concentin supports a fast, simple, and ultracentrifugation-free workflow
Simply add Retro-Concentin to retrovirus supernatant, incubate at 4°C overnight, and then spin at 1,500g for 30-minutes at 4°C. An optional spin in a microfuge tube at 12,000 rpm for 2 minutes can further concentrate the retrovirus particles.
Supporting Data
Retrovirus particles concentrated with Retro-Concentin are non-toxic and effective at transduction
Figure 1. Retrovirus particles concentrated with Retro-Concentin are non-toxic and effective at transduction. Transduced cells show normal morphology and with no obvious cytotoxicity, and were successfully used for generating induced pluripotent stem cells.
Resources
Citations
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- Gao, L, et al. (2017) Direct Generation of Human Neuronal Cells from Adult Astrocytes by Small Molecules.. Stem Cell Reports.2017 Mar 14; 8(3):538-547. PM ID:28216149
- Ye, L, et al. (2017) mTOR Promotes Antiviral Humoral Immunity by Differentially Regulating CD4 Helper T Cell and B Cell Responses. J. Virol..2017 Feb 15; 91(4). PM ID:27974559
- Wang, H & Sun, W. (2017) CRISPR-mediated targeting of HER2 inhibits cell proliferation through a dominant negative mutation. Cancer Lett..2017 Jan 28; 385:137-143. PM ID:27815036
- Farkašova, H. (2017) Host-virus interactions of mammalian endogenous retroviruses. Thesis.;. Link:Thesis
- Schnittke, N, et al. (2015) Transcription factor p63 controls the reserve status but not the stemness of horizontal basal cells in the olfactory epithelium. Proc. Natl. Acad. Sci. U.S.A..2015 Sep 8; 112(36):E5068-77. PM ID:26305958
- Habib, O, et al. (2014) Activation-induced deaminase-coupled DNA demethylation is not crucial for the generation of induced pluripotent stem cells. Stem Cells Dev..2014 Feb 1; 23(3):209-18. PM ID:24083501
- Coussens, NP, et al. (2013) Multipoint binding of the SLP-76 SH2 domain to ADAP is critical for oligomerization of SLP-76 signaling complexes in stimulated T cells. Mol. Cell. Biol..2013 Nov 1; 33(21):4140-51. PM ID:23979596
- Miller, LK, et al. (2013) Proteasome inhibitors act as bifunctional antagonists of human immunodeficiency virus type 1 latency and replication. Retrovirology.2013 Oct 24; 10:120. PM ID:24156270
- Lin, S, et al. (2012) Caveolin-1 reduces HIV-1 infectivity by restoration of HIV Nef mediated impairment of cholesterol efflux by apoA-I. Retrovirology.2012 Oct 15; 9:85. PM ID:23067370
- Scheideman, EH, et al. (2012) Transmembrane protein aptamers that inhibit CCR5 expression and HIV coreceptor function. J. Virol..2012 Oct 1; 86(19):10281-92. PM ID:22811524
- Musselman, CA, et al. (2012) Bivalent recognition of nucleosomes by the tandem PHD fingers of the CHD4 ATPase is required for CHD4-mediated repression. Proc. Natl. Acad. Sci. U.S.A..2012 Jan 17; 109(3):787-92. PM ID:22215588
