4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
System Biosciences(授权代理)
主营:RNA干扰(RNAi)研究之相关工具
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > RNA_interference > SBI/Retro Concentin™逆转录病毒浓度试剂/100 mL/RV100A-1-100 mL
商品详细SBI/Retro Concentin™逆转录病毒浓度试剂/100 mL/RV100A-1-100 mL
SBI/Retro Concentin™逆转录病毒浓度试剂/100 mL/RV100A-1-100 mL
SBI/Retro Concentin™逆转录病毒浓度试剂/100 mL/RV100A-1-100 mL
商品编号: RV100A-1-100mL
品牌: System Biosciences
市场价: ¥6580.00
美元价: 3948.00
产地: 美国(厂家直采)
公司:
产品分类: RNA干扰
公司分类: RNA_interference
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

Increase the infectivity of any retrovirus by concentrating with Retro-Concentin

Quantitatively precipitate any retrovirus with Retro-Concentin™. Instead of using lengthy spins in an ultracentrifuge, Retro-Concentin enables concentration of retroviruses directly from culture medium with a simple one (or an optional two) low-speed centrifugation steps.

In addition, Retro-Concentin stabilizes the retroviruses for frozen storage, providing an additional advantage over other concentration methods.

Each preparation can handle up to 200 mL of retroviral supernatant, which is compatible with most centrifuges, and the resulting pellet can be dissolved in the volume of buffer that meets your experimental requirements.

With Retro-Concentin, you get simple and effective retrovirus concentration:

  • Fast protocol
  • Cost-effective
  • Up to 100-fold concentration of retrovirus particles

How It Works

Retro-Concentin supports a fast, simple, and ultracentrifugation-free workflow

Simply add Retro-Concentin to retrovirus supernatant, incubate at 4°C overnight, and then spin at 1,500g for 30-minutes at 4°C. An optional spin in a microfuge tube at 12,000 rpm for 2 minutes can further concentrate the retrovirus particles.

Supporting Data

Retrovirus particles concentrated with Retro-Concentin are non-toxic and effective at transduction

Figure 1. Retrovirus particles concentrated with Retro-Concentin are non-toxic and effective at transduction. Transduced cells show normal morphology and with no obvious cytotoxicity, and were successfully used for generating induced pluripotent stem cells.

Resources

User Manual: Retro-Concentin
Product Sheet: Retro-Concentin Retrovirus Concentration Reagent
Brochure: Gene Delivery and Expression Products and Services

Citations

  • Kobayashi, Y, Gélinas, C & Dougherty, JP. (2017) Histone deacetylase inhibitors containing a benzamide functional group and a pyridyl cap are preferentially effective human immunodeficiency virus-1 latency-reversing agents in primary resting CD4+ T cells. J. Gen. Virol..2017 Apr 1; 98(4):799-809. PM ID:28113052
  • Gao, L, et al. (2017) Direct Generation of Human Neuronal Cells from Adult Astrocytes by Small Molecules.. Stem Cell Reports.2017 Mar 14; 8(3):538-547. PM ID:28216149
  • Ye, L, et al. (2017) mTOR Promotes Antiviral Humoral Immunity by Differentially Regulating CD4 Helper T Cell and B Cell Responses. J. Virol..2017 Feb 15; 91(4). PM ID:27974559
  • Wang, H & Sun, W. (2017) CRISPR-mediated targeting of HER2 inhibits cell proliferation through a dominant negative mutation. Cancer Lett..2017 Jan 28; 385:137-143. PM ID:27815036
  • Farkašova, H. (2017) Host-virus interactions of mammalian endogenous retroviruses. Thesis.;. Link:Thesis
  • Schnittke, N, et al. (2015) Transcription factor p63 controls the reserve status but not the stemness of horizontal basal cells in the olfactory epithelium. Proc. Natl. Acad. Sci. U.S.A..2015 Sep 8; 112(36):E5068-77. PM ID:26305958
  • Habib, O, et al. (2014) Activation-induced deaminase-coupled DNA demethylation is not crucial for the generation of induced pluripotent stem cells. Stem Cells Dev..2014 Feb 1; 23(3):209-18. PM ID:24083501
  • Coussens, NP, et al. (2013) Multipoint binding of the SLP-76 SH2 domain to ADAP is critical for oligomerization of SLP-76 signaling complexes in stimulated T cells. Mol. Cell. Biol..2013 Nov 1; 33(21):4140-51. PM ID:23979596
  • Miller, LK, et al. (2013) Proteasome inhibitors act as bifunctional antagonists of human immunodeficiency virus type 1 latency and replication. Retrovirology.2013 Oct 24; 10:120. PM ID:24156270
  • Lin, S, et al. (2012) Caveolin-1 reduces HIV-1 infectivity by restoration of HIV Nef mediated impairment of cholesterol efflux by apoA-I. Retrovirology.2012 Oct 15; 9:85. PM ID:23067370
  • Scheideman, EH, et al. (2012) Transmembrane protein aptamers that inhibit CCR5 expression and HIV coreceptor function. J. Virol..2012 Oct 1; 86(19):10281-92. PM ID:22811524
  • Musselman, CA, et al. (2012) Bivalent recognition of nucleosomes by the tandem PHD fingers of the CHD4 ATPase is required for CHD4-mediated repression. Proc. Natl. Acad. Sci. U.S.A..2012 Jan 17; 109(3):787-92. PM ID:22215588
Search all Citations
品牌介绍
美国SBI代理 System Biosciences,简称SBI,美国加州湾区新成立的生技公司,致力于独特、创新生物技术之开发,以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰(RNAi)研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术,为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司,提供基于慢病毒 美国SBI代理 ExoQuick and ExoQuick-TC Products Product Size Catalog # ExoQuick serum exosome precipitation solution (5 ml) 75 reactions EXOQ5A-1 ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product 75 reactions EXOQ5TM-1 Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product 100 reactions TMEXO-1 ExoQuick serum exosome precipitation solution (20 ml) 300 reactions EXOQ20A-1 ExoQuick-TC for Tissue Culture Media and Urine (10ml) 10 reactions EXOTC10A-1 ExoQuick-TC for Tissue Culture Media and Urine (50ml) 50 reactions EXOTC50A-1 Exosome-depleted FBS Media Supplement - USA Certified 50 ml EXO-FBS-50A-1 Exosome-depleted FBS Media Supplement - USA Certified 250 ml EXO-FBS-250A-1 ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit 5 reactions EXOLP5A-1
联络我们
服务热线:4000-520-616
(限工作日9:00-18:00)
QQ :1570468124
手机:18915418616