Overview
Accelerating in vivo genome editing
From basic biological studies to generating novel disease models, SBI’s injection- and transfection-ready PrecisonX™ Cas9 SmartNuclease™ mRNA is here to move your in vivo genome engineering projects forward faster.
Use with an appropriate gRNA and, if your application needs it, a homologous recombination (HR) targeting vector for gene knock-ins, knock-outs, editing, and tagging. The Cas9 SmartNuclease will generate a double-strand break (DSB) at the site specified by the gRNA, and this DSB can be efficiently and precisely repaired in a manner that includes your desired mutation through the use of an HR targeting vector.
As with all of our Cas9 delivery options, the Cas9 SmartNuclease mRNA is functionally validated and comes backed by our expert technical support team—if you’ve got a genome engineering question just ask by emailing tech@systembio.com.
- Ready to use for in vivo genome editing applications
- Functionally validated Cas9 SmartNuclease
- Backed by expert, easy-to-reach technical support
Why an HR targeting vector is a recommended
Even though gene knock-outs can result from DSBs caused by Cas9 alone, SBI recommends the use of HR targeting vectors (also called HR donor vectors) for more efficient and precise mutation. HR donors can supply elements for positive or negative selection ensuring easier identification of successful mutation events. In addition, HR donors can include up to 6-8 kb of open reading frame for gene knock-ins or tagging, and, when small mutations are included in either 5’ or 3’ homology arms, can make specific, targeted gene edits.
Efficiently generate gRNA with the T7 gRNA SmartNuclease Synthesis Kit
Pair your Cas9 SmartNuclease mRNA with our T7 gRNA SmartNuclease Synthesis Kit, which comes with a pre-linearized, ready-for-cloning gRNA expression vector and all the reagents you need for T7-driven in vitro transcription of your cloned gRNA.
Not sure whether you need a CRISPR/Cas9 plasmid, purified protein, or mRNA?
Use this table to choose the CRISPR/Cas9 product that’s right for you:
For This Application | In these types of cells |
|---|
Use These Products
- Gene tagging
- Transgenic organism generation
- Model organism engineering
- Stable KO, KI, and genome editing of somatic cells
- Transgenic cell line generation
- Cell-based disease models
- Primary cells
- Hematopoietic cells
- Stem cells
- Genome-wide surveys
- gRNA library screens
- Functional screens
- Off-target events are of highest concern
