Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-MSCV-MCS-EF1α-GFP Cloning and Expression Lentivector drives expression of your gene-of-interest from the MSCV promoter, which provides strong expression in hematopoietic and stem cells. This lentivector also enables selection for transductants using a GFP reporter.

With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:

• Multiple promoter options
• Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
• Coordinated co-expression via T2A or IRES
• Single promoter, dual promoter, and bidirectional promoter formats
• High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter

Not sure which promoter is right for you? This table should help:

Resources

Citations

• Shao, Y, et al. (2019) Overexpression of CXCR7 promotes mesenchymal stem cells to repair phosgene-induced acute lung injury in rats. Biomedicine & Pharmacotherapy.2019 Jan 1; 109:1233-1239. Link:Biomedicine & Pharmacotherapy
• Xu, L, et al. (2018) MiR-34c ameliorates neuropathic pain by targeting NLRP3 in a mouse model of chronic constriction injury. Neuroscience.2018 Dec 26;. PM ID:30593918
• Foerster, F, et al. (2018) Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells. Oncoimmunology.2018 Dec 26; 7(4):e1409929. PM ID:29632723
• Qi, S, et al. (2018) MCPIP1 mediates inflammatory responses induced by lipopolysaccharide and lipoteichoic acid in bovine mammary epithelial cells. Acta Biochim. Biophys. Sin. (Shanghai).2018 Dec 26;. PM ID:30590418
• Sekiba, K, et al. (2018) Pevonedistat, a first-in-class NEDD8-activating enzyme inhibitor, is a potent inhibitor of hepatitis B virus. Hepatology.2018 Dec 26;. PM ID:30586159
• Hino, N, et al. (2018) An amphipathic helix of vinexin α is necessary for substrate stiffness-dependent conformational change in vinculin. J. Cell. Sci..2018 Dec 21;. PM ID:30578314
• Ren, F, et al. (2018) The R132H mutation in IDH1 promotes the recruitment of NK cells through CX3CL1/CX3CR1 chemotaxis and is correlated with a better prognosis in gliomas. Immunol. Cell Biol..2018 Dec 21;. PM ID:30575118
• Xie, Y, et al. (2018) Molecular network of miR-1343 regulates the pluripotency of porcine pluripotent stem cells via repressing OTX2 expression. RNA Biol.2018 Dec 20;. PM ID:30567463
• Rostami, M, Haidari, K & Shahbazi, M. (2018) The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells. International Journal of Cell Biology.2018 Dec 19; 2018:1-14. Link:International Journal of Cell Biology
• Saeed, U, et al. (2018) Parvulin 14 and parvulin 17 bind to HBx and cccDNA and upregulate HBV replication from cccDNA to virion in a HBx-dependent manner. J. Virol..2018 Dec 19;. PM ID:30567987
• Vasjari, L, et al. (2018) Ras signals principally via Erk in G1 but cooperates with PI3K/Akt for Cyclin D induction and S-phase entry. Cell Cycle.2018 Dec 18;. PM ID:30560710
• Verhagen, HJMP, et al. (2018) IGFBP7 Induces Differentiation and Loss of Survival of Human Acute Myeloid Leukemia Stem Cells without Affecting Normal Hematopoiesis. Cell Rep.2018 Dec 11; 25(11):3021-3035.e5. PM ID:30540936
• Lee, PC, et al. (2018) Targeting PKCδ as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer. Cancer Cell.2018 Dec 10; 34(6):954-969.e4. PM ID:30537515
• Schoderboeck, L, et al. (2018) Genetic targeting and chemogenetic inhibition of new born neurons. Hum Gene Ther Methods.2018 Dec 7;. PM ID:30526082
• Gianni, M, et al. (2018) S100A3 a partner protein regulating the stability/activity of RARα and PML-RARα in cellular models of breast/lung cancer and acute myeloid leukemia. Oncogene.2018 Dec 7;. PM ID:30532072
• Shen, CY, et al. (2018) The extracellular SEMA domain attenuates intracellular apoptotic signaling of semaphorin 6A in lung cancer cells. Oncogenesis.2018 Dec 5; 7(12):95. PM ID:30518871
• Huang, Z, et al. (2018) Hsa-miRNA-29a protects against high glucose-induced damage in human umbilical vein endothelial cells. J. Cell. Biochem..2018 Dec 5;. PM ID:30520097
• Briard, B, et al. (2018) Fungal ligands released by innate immune effectors promote inflammasome activation during Aspergillus fumigatus infection. Nat Microbiol.2018 Dec 3;. PM ID:30510167
• Huang, YW, et al. (2018) A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder. Aging Dis.2018 Dec 1; 9(6):1043-1057. PM ID:30574417
• Briem, E, et al. (2018) MiR-203a is differentially expressed during branching morphogenesis and EMT in breast progenitor cells and is a repressor of peroxidasin. Mech. Dev..2018 Nov 30;. PM ID:30508578