Overview

Easy and consistent shRNA delivery and expression

Not just for genes, the PiggyBac system is also an excellent choice for reliably producing shRNA. The PB-CMV-GreenPuro-H1-MCS shRNA Cloning and Expression Vector (Cat.# PBSI505A-1) drives production of your shRNA from the strong H1 promoter. The vector also features GFP and puromycin resistance co-expressed from the strong CMV promoter, with co-expression mediated by the T2A element.

With the PiggyBac Transposon System, you can:

• Make transgenic cell lines with a single transfection
• Integrate multiple PiggyBac Vectors in a single transfection
• Insert an expression cassette into human, mouse, and rat cells
• Deliver virtually any-sized DNA insert, from 10 – 100 kb
• Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
• Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)

Customer AgreementsAcademic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology.For end user license information, see the following:

• Academic Customers—End-User License Information
• Commercial Customers—End-User License Information

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.

How It Works

The PiggyBac Transposon System’s Cut-and-Paste Mechanism

The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.

• The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
• The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
• The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal

Resources

Citations

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