Overview
Easily advance your study of miRNAs
With SBI’s Lenti-miR™ Pooled miRNA Precursor Virus Library, you can quickly and easily prepare cells that overexpress miRs for phenotypic screens. Our Lenti-miR Pooled miRNA Precursor Virus Library includes our complete collection of human miRs delivered as a pool of ready-to-transfect plasmid or a pool of high-titer lentivirus ready for immediate infection of cell lines, stem cells, and animal tissue/xenograft models. You get all of the benefits of our Lenti-miR™ technology as a screening-ready library.
After screening, you can easily identify which miR is overexpressed in a specific cell by performing a simple genomic PCR reaction using the included lentivector-specific primers, followed by direct sequencing. All miRNA precursor clones are about 500-700 bp in size.
Why choose SBI’s Lenti-miRs?
SBI’s comprehensive collection of Lenti-miRs—human precursor miRNA lentivectors—are a superior alternative to synthetic RNAs. Like synthetic miRs, SBI’s Lenti-miRs can be transfected into target cells for transient miR expression. But unlike synthetic miRs, they can also be transduced into a variety of target cells—including primary cells, stem cells, and other hard-to-transfect cell lines—to create stable miR-producing cell lines, maximizing your options for miR expression.
In addition, SBI’s Lenti-miRs are designed for efficient expression and accurate processing into mature miRNAs, for native miR-like behavior. Each construct is cloned with the native stem-loop structure plus an additional 200 – 400 bps of upstream and downstream DNA, ensuring that the miRNA processing machinery operates just as it would with the native transcript.
And because our collection includes cancer and stem cell-related miRNA clusters, we offer a wider range of constructs than just the mature miRNA sequences listed in miRbase.
The precursor miRNA Expression Lentivectors drive expression of the miR precursor with the constitutive CMV promoter, for strong expression in common cell types including HeLa, HEK293, and HT1080 cell lines. Transductants/transfectants can be easily sorted using copGFP.
- Transfect or transduce the precursor miRNA lentivectors
- Create stably-expressing miR cell lines
- Leverage SBI’s highly-regarded lentivectors
- Obtain accurate miR processing and efficient expression through the use of native precursor sequences
- Use copGFP and puromycin markers to identify desired clones
How It Works
Using the Lenti-miR Library
Screening with the Lenti-miR Library is straightforward
- Transduce the Lenti-miR Library into target cells
- Treat to induce phenotype
- Use a functional assay (invasion assay, differentiation assay, proliferation assay, apoptosis assay, etc.) to select for cells exhibiting the desired phenotype
- Identify miR effectors by amplifying effector miR and sequencing
SBI’s Human pre-miRNA Expression Lentivectors use the native miRNA processing machinery
