Overview
Easily track oncogenesis using qPCR
With qPCR primers for a carefully curated set of miRNAs involved in cancer, apoptosis, and differentiation, and SBI’s sensitive and reliable RNA-Quant™ cDNA Synthesis Kit, our OncoMir qPCR Array provides a streamlined system for studying oncogenesis with qPCR. The OnocoMir qPCR Array can simultaneously quantitate relative expression level differences between two or more samples for 95 separate markers, with all miRNAS carefully selected from the published literature.
- Simultaneously profile 95 different miRNAs known to be involved in apoptosis, differentiation, and cancer
- Identify miRNA biomarkers and expression pattern signatures
- Rapidly tag and convert all small RNAs into detectable cDNA for qPCR
- Measure as little as picogram amounts of starting total RNA
- Conduct high-throughput screens of clinical samples including biopsies and LCM sample
About the included RNA-Quant cDNA Synthesis Kit
With a single cDNA synthesis reaction, you can efficiently prepare all of the RNAs in your sample for real-time qPCR using the RNA-Quant cDNA Synthesis Kit. Unlike other cDNA synthesis kits which can only prepare one or two classes of RNAs for qPCR, the RNA-Quant Kit enables qPCR measurement of many classes of RNA from a single cDNA synthesis reaction. The kit includes three endogenous RNA reference qPCR assays as for human and mouse samples—miR-16, Y RNA lncRNA, and GAPDH mRNA.
An all-in-one cDNA synthesis kit
- Tail and tag all RNAs for cDNA synthesis
- Obtain full RNA coverage for any purpose
- Profile any RNA—mRNA, miRNA, lncRNA, sn/snoRNA, rRNA
How It Works
Using the OncoMir qPCR Array
First, prepare RNA for qPCR using the RNA-Quant cDNA Synthesis Kit
The poly-A tailing and tagging method in the RNA-Quant System generates cDNA that is highly unique and, when used with the 3’ Universal Reverse Primer, does not produce any signal in the absence of reverse transcriptase or lead to the detection of any residual genomic DNA. The result is zero background for RNA profiling with complete confidence.
- Tag all small RNAs with a poly-A tail
- Anneal an oligo-dT adaptor
- Reverse transcribe to create first-strand cDNA
Your final product will be a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.
Then conduct qPCR assays using the 96-well primer array
[table “” not found /]Get additional information about the array including miRNA sequences, miRbase accession numbers, and qPCR analysis templates in the Excel document, “Free Analysis Software for Cancer qPCR Array”
Supporting Data
Example user-generated OncoMir qPCR Array Data
