Nbsbio/Fluorescein labelling Kit - NH2 [3 samples]/LK01-10/1 Ea

来自 : 发布时间：2025-04-27

Details

Only 1 hour to recover conjugates

All processes in a single Filtration tube

High recovery of conjugates

Applicable for 50-200 μg IgG

Fluorescein Labelling Kit-NH2 is mainly used for the preparation of fluorescein-labelled proteins such as IgG for immunostaining and cellular proteins for tracing. Amine-reactive fluorescein, a component of this kit, has succinimidyl groups (NHS) that react with the amino groups on proteins or other molecules.This kit contains all the reagents necessary for labelling, including storage buffer. Each vial of fluorescein can label up to 200 μg of IgG, conjugating about 4 to 6 fluorescein molecules per IgG molecule. Because this kit also includes a buffer exchange system, a sample containing amine base buffer can be labelled. Although membrane filtration sometimes causes IgG aggregation, the buffer system in this kit prevents aggregation during the concentration of IgG or fluorescein-labelled IgG solution. A fluorescein-labelled IgG solution prepared using this kit is stable for more than 2 months at 4ºC. The excitation and emission wavelengths of the fluorescein labelled IgG are 495 nm and 520 nm, respectively.

---

Q&A

Can I use this kit for other proteins?Yes, if the molecular weight is greater than 50,000.Do I have to use a Filtration tube prior to labeling the protein? Product Code LK01-10 Cas # N/A Pack Size 3 samples Manufacturer Dojindo